Transformation efficiency
The transformation efficiency
is defined as the number of transformants generated per µg of supercoiled
plasmid DNA used in the transformation reaction.
Transformation efficiency is
calculated using the formula below-
= Number of Colonies on Plate X 1000 ng/µg
Amount
of DNA plated (ng)
Example 1 -
Number
of Colonies on Plate- 105,
Amount
of DNA plated- 0.1 ng
= 105 X 1000
ng/µg
0.1
= 1.05 X 106 cfu/µg
cfu-
colony forming units
Example 2 -
Number
of Colonies on Plate- 206,
Amount
of DNA plated- 0.1 ng
= 206
X 1000 ng/µg
0.1
= 2.06 X 106 cfu/µg
cfu-
colony forming units
